Abstract: The Shuiyindong gold deposit is located in the western section of Huijiabao antiline, and it is an important component of Huijiabao Carlin ore field. The orebodies occur in stratified or stratoid shape in high-porosity bioclastic limestone of the Upper Permian Longtan Formation within 300 m near the axis of the Huijiabao anticline. The shape of the orebodies is similar with that of the anticline. The main metal minerals are pyrite, arsenopyrite, realgar and orpiment. The gold mineralization related closely with silicification, dolomitization and pyritization. The geochemical study of isotopes indicates that the ore-forming materials mainly originated from plutonic source. Based on metallogenic condition of the Shuiyindong gold deposit, it is proposed that special tectonic background, favorable hosting rock assemblage and rich ore-forming materials and fluids are favorable conditions for gold mineralization. These are helpful for further understanding of Carlin gold mineralization, and can provide useful recommendations for reasonable exploration of gold resources in southwestern Guizhou Province.