Abstract: The oil and gas shows in the Jurassic of Ruoqiang sag of Tarim Basin are active, indicating significant exploration potential. However, the unclear characteristics of source rocks and oil-gas origins have constrained the evaluation of hydrocarbon resources and exploration breakthroughs in this area. By comprehensively utilizing field outcrops, well core samples, and seismic data, the researchers conducted a systematic analysis of the reservoir-forming conditions in the Jurassic system of Ruoqiang sag. The results revealed that the Jurassic system in Ruoqiang sag developed two sets of high-quality source rocks, including the lacustrine dark mudstone of Yangye Formation (the Middle Jurassic) and the carbonaceous mudstone coal-bearing strata of Kangsu Formation (the Lower Jurassic). Among these, Kangsu Formation source rocks exhibited high organic matter abundance, with an average total organic carbon (TOC) content reaching 7.6%, primarily composed of Type II₂-III humic kerogen and in low-mature to mature stage. Yangye Formation source rocks also had relatively high organic matter abundance (average TOC of 4.2%), dominated by Type II₂ humic kerogen, with thermal maturity ranging from immature to the early stage of the oil window. Ruoqiang sag featured three favorable oil and gas accumulation areas that is Ruoqiang, Jianggalesai, and Rubei recfonic belts. Among these, Ruoqiang tectonic belt was located at the center of Ruoqiang sag, coinciding with high TOC and high R_o zones. The source rocks had high abundance, moderate maturity and favorable tectonic positioning, making it the most favorable for hydrocarbon accumulation. Jianggalesai tectonic belt ranked second. This research could provide geological support for oil and gas exploration in the complex structural zones of Tarim Basin's southeastern foothills.