Abstract: The research on high-temperature geothermal resources in Guizhou is still in the exploratory stage. To study their formation conditions and occurrence patterns, based on regional geothermal exploration data, borehole temperature measurement data, and gravity and magnetic interpretation results, the authors in this paper have systematically analyzed the key influencing factors such as geothermal gradient, terrestrial heat flow, and reservoir-caprock combinations. The research indicated that the geothermal gradient in Guizhou generally featured higher values in the north and south and lower values in the east and west, with an average of 2.4 ℃/100 m. Significant thermal anomalies (3.0~3.5 ℃/100 m) were observed in Bijie-Jinsha in Northwestern Guizhou, Ziyun-Wangmo in Southwestern Guizhou, and Dejiang-Sinan in Northeastern Guizhou. The terrestrial heat flow values ranged from 30 to 125 mW/m², with high-value areas being distributed in the western part of Weining-Ziyun Fault and in Dejiang-Sinan region. The measured values in Weining mineral exploration area reached 52.8~94.4 mW/m². The Triassic to Cambrian clastic rocks constituted a regional low-thermal-conductivity caprock (thermal conductivity < 2.5 W/(m·K)), forming five sets of reservoir-caprock combinations with the underlying carbonate rock reservoirs. The Devonian-Carboniferous limestone reservoirs in Wangmo-Ceheng area in southwestern Guizhou were over 2000 m thick, providing excellent thermal storage conditions. This study comprehensively delineated three prospective areas of high-temperature geothermal resources in Guizhou Province, including Dafang-Jinsha area in Northwestern Guizhou (sedimentary basin type), Wangmo-Ceheng-Anlong area in Southwestern Guizhou (high radioactive type), and Weining-Liupanshui area in Western Guizhou (tectonic active zone type). The study predicted that the temperature could reach 150 ℃ at the depth of 7 100~7 900 m. The study clarifies the potential areas and formation conditions of high-temperature geothermal resources in Guizhou Province, providing some references for subsequent exploration target area optimization and development and utilization.