Abstract: The social development and urbanization process have a negative impact on the ecosystem of the black soil region in Northeast China, and accurate accounting of ecosystem service value (ESV) is of great significance to regional coordinated development. Lishu County of Jilin Province was taken as a study case and the land use type data of Lishu County during the period of 2000—2020 were set as basis. Then, the ESV was estimated by means of correction coefficients and consumer price index (CPI) by ArcGIS software, and the spatial evolution characteristics of ESV in Lishu County was researched by using 1 km×1 km grid. The results showed that during the 20-year period, the ESV in Lishu County increased and then decreased, with the highest value of 71.71×10⁸ yuan in 2010 and the lowest value of 24.47×10⁸ yuan in 2000. The five functional service categories of soil conservation, climate regulation, food production, gas regulation and hydrological regulation contributed the most among the value of all 11 individual ecosystem services. Spatially, the ESV per unit area in the central region of Lishu County was significantly lower than that in the west and southeast. The high-high aggregation areas were mainly distributed in the southeast and west of Lishu County, and the low-low aggregation areas were mainly distributed in the middle of Lishu County. The analysis found that it could effectively improve the evaluation accuracy of ESV by integrating the core indicators, such as corn planting area, yield and market price and by using the CPI index to correct the three-stage data from 2000 to 2020 for the temporal and spatial evolution of ESV in the study area. And it could provide more reliable data reference for land structure adjustment and ecological environment protection in black soil area.