Abstract: The study on the dynamic change of comprehensive regionalization of natural resources in North China can help researchers grasp the distribution law and evolution trend of natural resources in detail and provide technical support for the unified management and decision-making of natural resources. Based on the previous regionalization of grassland and cultivated land region in North China, the authors in this paper have selected the land surface natural resource classifications of forest, grassland, wetland, water resources and the closely related factors such as topography and climate, and adopted the grid assignment method to divide the 1990-2018 comprehensive regionalization into 11 secondary natural resource sub-regions and 18 tertiary natural resource regions. Besides, the distribution and dynamic changes of the grassland and cultivated land region natural resources in North China were analyzed on this basis. The results show that the main change of natural resources in the southern sub-region is the conversion of cultivated land to construction land from 1990 to 2018, while the main change of the some northern sub-regions is the conversion of grassland to forest, cultivated land, and desert. The further regionalization of natural resource region in North China is of great significance to the fine management of natural resources and the grasp of the dynamic characteristics of natural resources in typical regions in North China.